This study evaluated the gene expression profile of the human adipose‐derived stem cells (hASCs) grown on the Biosilicate®/F18 glass scaffolds. It demonstrated that gene expression of hASCs grown on the scaffold surface showed significantly increased gene expression related to hASCs cultured with the ionic extract or the osteogenic medium, evidencing that the BioS‐2P/F18 scaffolds have a substantial effect on cellular behavior of hASCs.